Of the dried powder of the aerial and root parts, 50 g was extensively extracted in Soxhlet extractor with methanol (500 ml) (HPLC grade, Rankem).
The beads were washed extensively, extracted with SDS sample buffer (10% glycerol, 1% SDS, 0.03% bromophenol blue, 62.5 mM TrisHCl, pH 6.8, 5% 2-mercaptoethanol) at 55°C, resolved by 10% acrylamide SDS-PAGE and transferred to Immobilon™ membrane (Millipore Corporation, Bedford, MA).
In doing this the credibility theory is extensively used to extract qualified crisp data from the available set of fuzzy results, so that standard optimizers can be adopted to solve the most important design problems.
A shape descriptor is numerical representation of a 2D or 3D shape in the form of vectors or graph data-structures and is extensively used to extract and describe features at visually important regions in the object.
First, for the purpose of avoiding competitive incorporation of canonical amino acids and UAAs, the cell extract was extensively washed using a diafiltration process to remove residual amino acids, thereby making the protein synthesis reaction completely dependent upon the exogenous addition of amino acids.
The beads were isolated from the extract and extensively washed, and recruited proteins extracted from the membrane were then analyzed by Coomassie blue-stained SDS-PAGE.
Regarding β-actin it is eye-catching that we can't see any reduction of this protein using EXB Plus, however we do detect lower β-actin levels in samples from extensively fixed tissues extracted with EXB.
Green tea (Camellia sinensis) is a famous herb, and its extract has been extensively used in traditional Chinese medicinal system.
We have extensively used this method to extract the lists of domain names from our selected TLDs.
So far, the advanced in situ leaching method has been extensively adopted to separate and extract ion-adsorbed REEs in southern Jiangxi Province, China (Wen et al. 2013).
