The most important implication for yeast physiology is that, our results being correct, cells within colonies continuously and extensively exchange anabolic metabolites among each other and do not, as the current assumption says, produce metabolites predominantly for themselves.
Prior to each measurement, enzyme was extensively buffer exchanged with 25 mM Tris (pH = 8.0) and 5% (v/v) glycerol.
The PfTR-positive fractions were confirmed by spectrophotometric and SDS PAGE analyses, pooled, and then extensively buffer exchanged by ultrafiltration with storage buffer [50 mM potassium phosphate, 500 mM NaCl, and 1 mM EDTA (pH 8.0)].
Purified proteins were extensively buffer exchanged into 25 mM sodium phosphate (pH 6.4), 5 mM NaCl, and 1 mM DTT. Peptide ligands were dissolved in the same buffer and estimated as described earlier; 50 μM protein in the sample cell was titrated against 1 mM peptide in the syringe at 30 °C in 1 μL increments at a stirring speed of 1000 rpm.
That SCC mec, metal resistance and SaPIn1 pathogenicity island elements are exchanged so extensively between clinical S. aureus and S. epidermidis isolates suggests that anthropogenic factors, such as antibiotic selective pressure, may affect genes in both species.
HAP column buffer was then exchanged and washed extensively with buffer D at 0.03 M KPi, pH 6.8 without KCl and NP-40.
The regional business was profitable because airlines used them extensively in exchange for a fixed fee per flight.
On county time, the staffers communicated extensively with Walker campaign staff, organized fundraisers, made invitations, exchanged fundraising lists, and sent out campaign press releases, all just steps away from Walker's own office.
During mammalian spermatogenesis, chromatin in differentiating germ cells is extensively remodeled, with the majority of nucleosomes being removed and ultimately exchanged by highly basic proteins named protamines.
