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An intrathecal injection is less invasive than an intralesional injection, can extensively deliver cells through the cerebrospinal fluid (CSF) [ 10] and is a more feasible routine for treatment of equine CNS injuries.
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Virtual reality has been used fairly extensively to deliver prolonged exposure therapy (PET) for PTSD since the 1990s, mostly for the treatment of soldiers and war veterans.
In the dorsal branch, which typically consists of five or six cells, one cell at the branch tip adopts the terminal cell fate and branches extensively to deliver oxygen to neighboring tissues whereas a second cell at the branch tip adopts a fusion cell fate and mediates fusion of tracheal branches from adjacent hemisegments and contralateral branches at the dorsal midline [13], [14].
The Tol2 transposon system has been used extensively to deliver exogenous DNA into the germline of zebrafish [ 1- 3], and more recently for successfully integrating BAC DNA into the germline of both zebrafish and mice [ 4].
Biomaterial scaffolds have been extensively used to deliver growth factors to induce new bone formation.
PEI is a synthetic cationic polymer that has been extensively used to deliver oligonucleotides, siRNA, and plasmid DNA in vitro and in vivo[8 10].
Both oil-in-water and water-in-oil emulsions have been extensively used to deliver drugs and cosmetic agents to the skin, depending on the property of active agents and the indications of the medicines.
Biomaterials have been extensively explored to deliver chemoattractants and create a physical microenvironment for recruited cells.
It has been extensively used to deliver many bioactive biomolecules across the BBB and into the CNS.
Therefore, it is not surprising that mRNA has been extensively exploited to deliver tumor antigens to these professional antigen-presenting cells (APCs).
Nontargeted, long-circulating liposomes, such as Doxil/Caelyx, have been extensively evaluated to deliver chemotherapeutic drugs to treat cancers via the enhanced permeability and retention mechanism [ 35– 35].
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