A well-established model system for oxytosis/ferroptosis is glutamate-induced cell death in the hippocampal cell line HT22, which has been used extensively to clarify the cascade leading to cell death and to identify antioxidant pathways and proteins (reviewed in).
Recognition of endotoxin or LPS, a major constituent of the outer membrane of gram-negative bacteria, has been extensively studied to clarify the mechanisms by which this component activates the immune system [1].
Highly virulent Yersinia enterocolitica strains have been extensively used to clarify the virulence/fitness mechanisms of this heterogeneous gastrointestinal pathogen.
The manuscript has been extensively rewritten to clarify the rationale for all of the experiments and to more effectively highlight the novel findings and conclusions.
In recent years, the role of PEEP has been extensively investigated to clarify its physiological effects in patients with ARDS and to define its role in protective ventilation strategies.
This is important because genome wide screening methods have been extensively used to clarify the role of sleep in brain function,, and current hypotheses on sleep function are based on changes in transcriptomes., Of course, more comprehensive studies examining a wider-array of genes, their proteins and activity-dependent transcription mechanisms (e.g., CREB-dependent transcription) are needed.
Especially using MTE, effects of nanoparticle treatment on cells must be clarified extensively.
Geographically-defined Primary Health Care areas were established on the basis of the characteristics of the regional organization for each primary health care system; these definitions were extensively discussed and clarified during the first meeting of the OTC SOCIOMED executive board to avoid variation of definition in terms of these characteristics across countries.
Over the years, details of energy production by tumors have been extensively investigated and clarified, unveiling that 'aerobic glycolysis' by no means implies that oxidative phosphorylation is shutoff in cancer cells, but rather that these cells may have a very active aerobic conversion of pyruvate to lactate, alongside with a normal oxidative phosphorylation.
As some examples: We extensively revised the text to clarify our presentation and our interpretations as requested.
