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Following extensive washing, cell-bound phage were eluted and used to infect TG1 cells.
After extensive washing the bound fractions were analyzed by western blotting.
Following extensive washing bound DG was eluted with the GST-SH3 domain using 20 mM glutathione.
After extensive washing the cover-slips were analyzed by a micro-imager to visualize radioactivity.
Following extensive washing, appropriate secondary antibodies were applied at a concentration of 1∶200.
The cartilage explants were fixed with 4% paraformaldehyde after protein transduction and extensive washing.
By extensive washing, the remaining non-adherent cells were removed.
After extensive washing, bound materials were eluted with Laemmli buffer and analysed by immunoblotting.
Extensive washing did not effect on the level of labelling on biotin kodecytes.
The reaction mixture was incubated for 1 h followed by extensive washing of beads with PBS.
After extensive washing, the cells were incubated with detection antibody and then Streptavidin-HRP.
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