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In addition, we found extensive staining for metastasis-associated protein 1 (MTA1).
The tissue expression of GFP/CTGF and α-SMA on days 0, 3 and 7 post-wounding was graded on a scale of 0 3 by three blinded observers; 0 signifies no staining, 1 signifies very little staining, 2 signifies moderate staining, 3 signifies extensive staining.
Using PAS, the rating was set for intensity and extension of staining, ranging from 0 (no staining) to 3 (intense and extensive staining), respecting tissue specificity scoring when adequate.
In contrast, the zfTTP1 morphants exhibited extensive staining throughout the body (Fig. 8d, 8h, 8l).
Probes derived from this fragment, however, generated very high levels of background, with extensive staining appearing on the exterior of the embryos within 15 minutes.
The SN neurons of the rotenone-treated rats also exhibited extensive staining with antibody against 8-oxo-dG, indicating oxidative modification of DNA (Figure 1C).
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In contrast, the irradiated cells showed extensive punctate staining of bright blue, as shown in the Figure 1(a).
Immunofluorescent staining of NRP1 and NRP2 showed extensive neointimal staining suggestive that NRP1 and NRP2 were at least partly localized to VSMC.
Benign tumours exhibited extensive epithelial staining with 6 out of 10 tumours exhibiting >50% of nuclear epithelial staining (Table 1, Figure 1B).
Similar to the Tg2576 murine model, PS1/APP mice showed extensive plaque staining [ 34, 35], but no significant difference in the retention of [C]PIB.
In both Cited2 −/flox;Wnt1Cre and in Cited2 +/flox;Wnt1Cre embryos, we observed extensive lacZ staining in the cranial mesenchyme, and in the first and second pharyngeal arches.
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