Lysosomal cysteine proteases similarly demonstrate broad specificity, and appear to be responsible for extensive proteolytic activity in this organelle [21].
However, it is clear from extensive enzymological surveys [ 22] that TyrAc proteins having broad specificity for NAD+/NADP+ are common, examples including species of Ralstonia and Burkholderia.
Quantitative real-time PCR assays are thought to be highly specific, and the findings of extensive analytical specificity studies performed in this and previous studies (14, 16) support this.
Extensive analytical specificity testing showed that among the 23 non- Aspergillus fungal species, our Aspergillus qPCR assay had significant cross-reactivity only with Penicillium chrysogenum and Paecilomyces variotii.
Similar to previous experiments on in vitro-adapted isolates [ 21], these ex vivo samples displayed extensive stage specificity in transcriptional regulation during their 48-hour IDC (Additional file 3).
It is worth noting the extensive village-specificity of the sub-clades.
In vertebrates, mitochondrial CYPs are generally specialized in the metabolism of steroid or vitamin D, in contrast with microsomal CYPs that show considerably extensive substrate specificities [ 1, 62].
In New Zealand, host-specificity testing is not currently formally regulated, but the Environmental Risk Management Authority ERMAA) is advising potential applicants of the importance of appropriate testing because approved applications to date typically included extensive host-specificity testing following a centrifugal phylogenetic approach (Barratt and Moeed 2005).
The 92 antibodies used in this study were carefully selected based on both their extensive validation for specificity as well as detecting key signalling molecules known for their involvement in motility, invasion, pro-survival, and growth factor signalling.
The almost perfect co-localization of fluorescence across all layers of the barrel cortex demonstrates the extensive sensitivity and specificity of this mouse line.
