But because it entails extensive sequencing, this approach comes with a higher price tag.
The taxonomic composition of the microbiome can be profiled using the marker-based approach, i.e., a PCR-amplification with universal primers of a taxon-specific gene, followed by the extensive sequencing of the amplicon by the preferred platform (Table 1).
Thus, we carried out extensive sequencing to construct a single contig.
A more confident analysis would require much more extensive sequencing data, or the use of microarrays.
The full-length cDNA was verified by extensive sequencing to ensure that no error had been introduced.
[29], [30] In principle, these limitations can be overcome by extensive sequencing of small RNA libraries from a broad range of samples.
We propose that with extensive sequencing the propensity for false positive miRNA identification increases dramatically if low abundance siRNAs are considered.
To explore gene variability in our population, an extensive sequencing of the VEGF gene was carried out in a total group of 136 individuals.
Focusing on the 6p21.1 signal, an extensive sequencing analysis of the VEGF gene was conducted in sub-samples from each of the three villages.
Extensive sequencing efforts suggest that natural antisense transcripts are prevalent throughout the mammalian genome; however, their biological significance has not been well defined.
In conclusion, after performing extensive sequencing of SLC2A4, we determined that it has a peculiar pattern of genetic variation, with the first part of the gene showing common and rare variants in a fashion compatible with neutral evolution.
