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Although use of more extensive sequences in the studies may have better characterized the identified abnormalities, they may also have identified additional abnormalities, so that the true prevalence of such lesions may be underestimated.
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This goal would require extensive sequencing in diverse populations and could become reachable in the next few years with the rise of next generation sequencing methods (Harismendy et al. 2009; Rokas and Abbot 2009).
The demonstration of more extensive sequence diversity in buffalo-derived parasites than in those maintained in cattle is consistent with the idea that a limited subset of the T. parva population has become adapted for maintenance by tick transmission in cattle, or that many parasite genotypes cannot be maintained in the cattle tick transmission cycle.
Extensive sequence differences in the hairpin and in the mature sequences of mir-64 paralogs suggest possible subfunctionalization following their origin by duplication (fig. 7 B), although conservation of the seed in all members implies an overlapping set of target genes.
For RMP species there is evidence that differences in virulence are associated with differences in RBC invasion, and, indeed, we find extensive sequence polymorphism in P. chabaudi genes encoding proteins involved in RBC invasion.
This also underlines, however, that near complete variant detection requires extensive sequence coverage in order to overcome variability in coverage depth across target regions.
In our recent paper [ 19], we demonstrated extensive sequence diversification in the numerous Abpa/Abpbg paralogues we predicted.
Nevertheless, we also showed extensive sequence divergence in the hairpin and mature sequences of mir-64 paralogs in both C. remanei and C. latens.
Alignments demonstrated extensive sequence variation in the V4 hypervariable region of the 18S rRNA gene within T. equi.
The recent availability of extensive sequence information in fish, however, has facilitated the application of more extensive molecular (including whole-genome) approaches to fish toxicology.
Noroviruses are important agents of human gastroenteritis characterized by extensive sequence variation in the major capsid structural protein that likely encodes critical antigenic determinants of protective immunity.
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