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Currently, most large-scale TCR-seq uses Illumina sequencers, which can generate extensive sequence data at low cost, although other platforms, in particular 454, have also been used successfully for smaller-scale analyses.
Here we demonstrate the power of matK for fern phylogenetic reconstruction, as well as provide primers and extensive sequence data that will greatly facilitate future evolutionary studies of ferns.
We argue that our model derivation procedure is immediately applicable to other organisms with extensive sequence data available, such as Hepatitis C and Influenza A viruses.
In this study, we present extensive sequence data from a total of 1090 recombinant H. pylori clones resulting from transformation experiments with different combinations of recipients and donors, and investigated the roles of a large set of DNA recombination and repair genes in determining the length of imports after natural transformation.
No Triticeae species have been fully sequenced, but there is extensive sequence data available from wheat, barley, and rye.
The utility of any species for comparative transcriptomic and genomic studies depends on its relationship to other taxa for which extensive sequence data are available.
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Conversely, little is known regarding the role and/or clinical impact of KRAS mutations in the setting of the hematological malignancies, including chronic lymphocytic leukemia (CLL), and only in recent years extensive sequencing data have highlighted the recurrent mutations of genes affecting the Ras MAPK pathway in CLL [2, 3].
A more confident analysis would require much more extensive sequencing data, or the use of microarrays.
Taxonomists would then combine extensive sequencing data with their morphological and ecological analysis, assisted by new technologies in digital imaging and web-based delivery (e.g. www.antweb.org and www.barcodinglife.org), to infer species limits and frame evolutionary context for species.
This strategy should be applicable for the elucidation of regulatory pathways in other systems by using the extensive sequencing data currently being generated.
However, a number of reports have provided both insights and tools for further study including extensive sequencing data, reverse genetic resources, and monoclonal antibodies for treatment of infection (Zumla et al., 2015).
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