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Medium was changed every 3 days for 10 14 days to allow extensive outgrowth.
The lower GABA concentrations seen in the cultured B1 neurons seem to be in accord with extensive outgrowth formation.
After 4 weeks, extensive outgrowth of nestin-positive bi- or tripolar cells migrating out of the explant was observed in each culture (Fig. 1b, c).
Similarly, 12-week-old pregnant (14.5dpc) Postn-deficient females displayed extensive outgrowth and arborization that was similar to wild-type or Postn littermates.
The transplantation of bone marrow stromal cells for spinal cord injury produced extensive outgrowth of regenerating axons associated with Schwann cells through the extracellular matrices [ 8, 19].
When grown on laminin coated substrate eight out of 11 small cell lung cancer (SCLC) cell lines exhibited attachment to laminin and three had extensive outgrowth of long neurite-like processes.
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Contamination of this luminal subpopulation (double-sorted and purity confirmed by reanalysis) with MaSCs seems unlikely as no outgrowths were evident in the absence of Matrigel, and no extensive outgrowths were ever observed.
In 2D cultures, the native brain ECM molecules promoted extensive neurite outgrowth and increased neuronal viability7,9.
In an extremely precise format, progenitor neurons migrate to targeted coordinates from different origins and elaborate extensive neurite outgrowth to allow the wiring of brain circuits2.
In addition, TuJ1+ neurons in RWV culture give rise to extensive neurite outgrowth and considerably more synapsin I+ pre-synaptic puncta surrounding MAP2+ cell bodies and dendrites.
Pre-NaBt treatment potentiated the neuritogenic activity of dbcAMP in a dose-dependent manner (Fig. 4a, b) by promoting extensive neurite outgrowth after 5 days of dbcAMP exposure, a response that dbcAMP alone could not trigger (Fig. S4A).
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