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The structure reveals a dimeric assembly created by an extensive network of interactions among the kinase, ubiquitin-like, and scaffold/dimerization domains.
As discussed below, this extensive network of interactions tunes the properties of active site residues, and distributes the energetic costs over the rest of the FeSOD structure.
The two short motifs were found to provide an extensive network of interactions and structural constraints that are used to keep a catalytic arginine residue in its highly constrained and optimal position.
Investigation of the ligand protein interactions of each, in particular the hydrogen bonding, reveals a more extensive network of interactions in 1NLY and the A chain also includes the active site metal (Mg).
These C-terminal structural changes allowed the formation of an extensive network of interactions between amino acid residues Tyr221, Arg223, and Glu224 of helix-3 and the backbone of amino acid residues 160, 170, 172, and 173 within the β-strand 2-helix-1 loop.
Whereas tri-alanine was observed adopting a vertical position and coordinated by 4 hydrogen bonds, the di-peptide L-Ala-L-Phe was held in a more horizontal position and coordinated through a more extensive network of interactions involving electrostatic interactions with conserved side chains from the N- and C-terminal bundles (Lyons et al., 2014).
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A host of analogous systematic detection approaches have now begun to chart the extensive networks of interactions and associated protein modifications in human cells [ 10].
They are organized through extensive networks of interaction, by tightly bound macromolecular assemblies or more transiently via signaling nodes.
These hydrophobic interactions are supported by an extensive network of polar interactions involving Arg153, Glu155, Thr166, Lys170, Lys221 and Glu223 from Sgt1, and Ser89, Asn93, Asp144, Asp145 and Glu146 from Hsp90.
An extensive network of lipid protein interactions mimicking native membranes is established and maintained in two-dimensional (2D) crystalline vesicles used for structural analysis by electron crystallography.
The close contact between these three domains is maintained by several hydrogen bonds and an extensive network of hydrophobic interactions.
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