Specificity of the assay has been shown by free ligand competition and extensive correlations with other immunological parameters.
While correlations were obtained with BMI and all of the metabolic parameters, the strongest and most extensive correlations were with AG levels.
Whereas the analyses started with 40 descriptors, based on extensive correlation and selectivity analyses, a subset model based on 18 descriptors was performing better than the model comprising all 40 descriptors.
Both Rnap2 ChIP-seq replicates from all three tissues exhibited extensive rank correlation with gene RPKMs (0.78 in liver, 0.70 in brain, and 0.77 in muscle), suggesting that our Rnap2 ChIP-seq data at promoters is an accurate predictor of gene expression levels in the same tissue.
We observed that, at a coarse-grain, the bulk of physical distances among AgMNPV-2D genes, expressed in map units, did not show extensive positive correlation with Euclidean distances among expression profiles of each individual ORF (Additional file 7: Figure S4), indicating that the association of gene expression profile is not explained by the tandem organization of ROTs alone.
Extensive correlations of OPN genotype with OPN protein levels have been previously reported for DMD patient muscle, and there was no association of genotype with OPN levels (24).
In addition to these regions, we found that the PMC has extensive functional correlations with other bilateral cortical areas within the frontal lobe, such as the VMPFC and the motor/supplementary motor cortex.
Finally, the HORMONOMETER analysis evidenced an extensive anti-correlation, with the only exceptions of all TFs and sTFs in the second and third comparisons, respectively.
Limitations of this study include the relatively small number of observers, the use of a highly selected group of patients (all with adult scoliosis severe enough to require extensive surgery), and no correlation with clinical symptoms.
