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Similarly, the color intensity profiles of the Alcian blue and Sirius red-stained images clearly demonstrated the formation of a fibrous collagenous band along the periphery of the pellet, evidenced by extensive collagen (intense red stain) production in comparison to PG (blue stain) production (Fig. S7).
Electron microscopy showed extensive collagen disorganization, decreased quantity of collagen, collagen fibril shrinkage, and chondrocyte damage when compared with a control portion.
In the early stages of OA, prior to extensive collagen damage, it may be possible to retard or even reverse the degenerative process by the administration of agents that promote aggrecan synthesis and prevent its degradation.
In the final phase, the wound scar tissue is remodeled by replacement of the provisional ECM with a stronger, durable ECM, characterized by extensive collagen cross-linking and the gradual replacement of type III collagen with type I collagen [125-127].
Fibrosis resulted in extensive collagen deposition (Sirius red) and increased distance between myocytes (Fast green) 6 weeks after infarction.
Confocal immunofluorescence analysis demonstrated the presence of extensive collagen IV protein structures that completely ensheathed EC networks at 72 hours (Figure 5A-B).
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With fibroblasts, the more extensive the collagen damage, the more contraction of the collagen occurred, and the greater was the decrease in collagen production.
The intra-lamellar spaces on the adventitial aspect of vessels exhibited the most extensive hypertrophy, collagen deposition and macrophage accumulation.
This laminar model of the ventricular myocardium views cardiac myocytes as being organized in branching layers surrounded by an extensive perimysial collagen network.
Cell cell interactions also influenced the aligned architecture, as extensive astrocyte-collagen contraction was achieved at high (9 12 × 105 cells/mL) but not lower (2 6 × 105 cells/mL) seeding densities.
Restoration of adnexa, and extensive fibrosis and collagen tissue within the dermis observed.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com