More prolonged treatment (30 min) did have an inhibitory effect; however, it also resulted in a ten-fold decrease in the extent of RTX opsonisation, suggesting that extensive cholesterol depletion prevents efficient RTX binding to CD20 (data not shown).
Use of a dominant-negative mutant of STARD3 (ΔSTART-STARD3) caused extensive cholesterol accumulation in CHO and COS-7 cells, accompanied by inhibition of late endosomal trafficking, similar to the phenotype caused by functional loss of NPC1/2 proteins [ 23].
In contrast, although fluorescence was detected within the cytosol, the intense plasma membrane fluorescence generated by filipin binding was greatly reduced by MβCD, indicating extensive and selective cholesterol depletion from the cell surfaces (Fig. 3B).
Treatment of HeLa cells with HPβCD, which also promotes extensive release of cholesterol from cells, reduced C. jejuni internalization in a dose-dependent manner (Additional file 3: Figure S3).
Pat Potocny, a 53-year-old high school math teacher in Philadelphia, has high cholesterol and extensive heart disease in her family.
Following the discovery of the link between NPC disease and cholesterol [20], extensive studies were pursued on CHO cells exhibiting an NPC phenotype and on human NPC mutant fibroblasts [2], [3], [8], [15], [21], [22], [23].
The assessment of total and particularly HDL cholesterol is more extensive and in case of cholesterol ratio requires two measurements.
Although Cis-regulatory regions might be the cause of LDL-cholesterol variability; an extensive analysis of the LDLR distal promoter has not yet been performed.
All the story also demonstrates that even in topic areas (high cholesterol, hypertension, diabetes) with extensive amounts of data and published clinical trials, crucial evidence is still missing.
Extensive tests analyzing factors that included blood cholesterol as well as noise exposure, the scientists found hearing loss among the musicians no worse than among the general population.
The human cDNA sequence predicts a 1278aa protein with a lysosomal targeting sequence, several trans-membrane domains and extensive homology with other known mediators of cholesterol homeostasis.
