We identified extensive cell type-specific expression changes: 6711 genes and 10,724 transcripts, enriched in non-protein-coding elements at early stages of differentiation.
Consistent with the hypothesis of extensive diversity in a single cell type, Friedländer et al. recently detected a total of 213 known miRNAs in a single HeLa sample [ 46].
An alternative approach for quantification of the PS's intracellular localization could involve organelle-specific fractionation, for example, by centrifugation techniques and analysis of the organelle-bound PS fluorescence this approach is more time-consuming and may require more extensive optimization for the particular cell type.
The expansion and diversification of the KLF/SP gene family within the Metazoa may thus reflect the accumulation of differential transcriptional repression strategies associated with the development of extensive repertoires of cell types required to support complex tissues.
Many areas require further study at the population level including detailed probing of extensive tissue and cell types, and ascertainment of QTLs related to splicing [ 4, 24], RNA decay mechanisms [ 81], non-coding RNA [ 76, 82], and epigenetic mechanisms such as methylation [ 28, 83– 83].
To further determine whether DNase I hypersensitivity mapping can discover regulatory elements associated with expression of genes specific to particular regions of the CNS, we focused on an analysis of the neural retina, for which there has been extensive characterization of cell types and gene expression [ 37, 38].
Extensive clinical data, including tumor histologic cell type, pathologic stage at presentation, and treatment, were recorded.
Extensive studies in mice also show cell type specific, non-random chromosome arrangements based on both gene density and chromosome size [4].
Embryonic stem cells (ESCs) have the potential to indefinitely self-renew and differentiate into any cell type [ 1], and extensive studies have been carried out to take advantage of these unique characteristics including tissue regeneration and repair [ 2- 5].
We observe extensive change in TF colocalizations both within a cell type exposed to different conditions and across multiple cell types.
