Furthermore, depletion of Ubc9 is difficult to establish in tissue culture and results in extensive cell-cycle defects and loss of cell viability (Boutell et al., 2011).
However, 11-oxo-treated cells were elongated and stretched, which could be indicative of extensive cytoskeletal remodeling, cell cycle arrest and/or differentiation.
While there has been extensive research on cell cycle mechanisms and cyclin function in animals and yeasts, only a small number of plant cyclins have been characterized functionally.
The microtubule cytoskeleton forms the most prominent structural system in Trypanosoma brucei, undergoing extensive modifications during the cell cycle.
B-MYB therefore exerts extensive control over the cell cycle, whereas the effects of FOXM1 are limited to G2-/M-phase gene expression (Martinez & DiMaio, 2011).
Aurora B protein is subject to extensive posttranslational modification throughout the cell cycle.
End-joining of uncapped telomeres occurs primarily in G1, a stage of the cell cycle where extensive resection is suppressed.
There has been considerable controversy regarding the ability of histone modifications to provide stable and heritable epigenetic information, given the extensive nucleosome dynamics throughout the cell cycle.
The abovementioned morphological alterations combined with the H4K20Me1 staining pattern provided an initial indication that PR-SET7 deficiency has little effect in non-dividing cells, but induces cell cycle arrest, extensive DNA damage and necrosis only in dividing cells.
The time-course of apoptotic initiation extended beyond one mitotic cell cycle and was usually accompanied by extensive phagocytosis on E13.
For extensive reviews regarding pRb and the cell cycle see [10, 11].
