The CD44 gene undergoes extensive alternative splicing of its variable exons, leading to the generation of numerous alternatively spliced isoforms.
In addition, we report extensive alternative splicing defects in the brains of BPD subjects compared to controls.
In rNav1.3 or hNav1.3, extensive alternative splicing sites, such as 12v1, 12v2 and 12v3 (rat), as well as 12v1, 12v2, 12v3 and 12v4 (human) in DI-II intracellular loop were identified to be important for channel kinetics (Thimmapaya et al., 2005).
This is in contrast to the extensive alternative splicing that exists in other honeybee neuroligins and NrxI [1].
Secondly, the α/β-hydrolase fold that this domain adopts is notoriously flexible with respect to accommodating extensive alternative splicing, insertions and deletions, and still giving rise to viable protein [56].
As mentioned above, we believe the long standing and hitherto unexplained complexity of the dumpy gene can best be explained by extensive alternative splicing where dpolv nonsense mutations tag constitutive exons presumably located at several different places in the gene.
While this is not certain to cover the full spectrum of all possible splice variants in all tissues, it highlights the fact that more extensive alternative splicing does take place and will affect conclusions made without knowledge of isoform architecture.
The exons in the 5' and 3' regions of CD44 are constitutively spliced, while ten exons in the middle of the gene undergo extensive alternative splicing in various tissues and cancers.
The leukocyte specific transcript-1 is a gene with extensive alternative splicing and is encoded within the TNF region of the HLA complex, and plays a role in inflammatory and infectious diseases [17], [18].
CD44, the receptor for hyaluronan [1], is encoded by a single gene on chromosome 11p13, but actually represents a polymorphic group of transmembrane glycoproteins owing to extensive alternative splicing and post-translational modifications [2].
Instead, the validity and biological relevance of coordinated gene expression typically relies on extensive alternative forms of conformation through combining datasets and through permutation testing procedures (both performed here) [13], [17], while the larger accuracy of gene networks has been confirmed through functional convergence across groups of affected genes [13] [16].
