The 167 bp amplicon was further extended in a second PCR reaction using oligonucleotides that in their 5′ extensions introduce Illumina adaptors and sample-specific barcodes (Table S3) for multiplexing up to 32 samples in one run of a MiSeq instrument.
The second extension introduced by Zirnstein et al.
The first extension introduced a dummy for late adopter which was defined as the municipalities which introduced the reform after half of the others had done so.
The opening of the Woodlands Extension introduced bolder pieces of artwork, such as a 4,000 kg sculpture in Woodlands.
The model extension introduced 17 new parameters and these needed to be set such that model results corresponded to data from available experimental perturbations, i.e. mutants and lung cultures.
Since this extension introduces nonlinear relationships, the problem is defined as a mixed integer nonlinear program (MINLP-model).
Pandora, one such extension, introduces temporal gates and temporal laws to allow dynamic analysis of temporal fault trees (TFTs).
This extension introduces covariance among the A, C, and E, and necessarily alters the meaning of the latent environmental variables, as we point out below.
The tracing rules also reveal that the extension introduces GE covariance, i.e., the additive genetic variables (A; at t = 2,…,T), and the unshared environmental influences (E; at t = 2,…,T) are correlated.
A Google spokeperson has described these "informative visual elements" as simply the latest progression in content-rich ads, building on services such as Image Extensions (first introduced in June 2013) that pairs text adverts with approved pictures.
