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The center of the NPC is thought to be filled by unstructured, highly repetitive protein extensions characterized by phenylalanine-glycine dipeptides (FG repeats), which are found in ∼1/3 of all nups (Rout et al., 2000; Cronshaw et al., 2002).
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B. subtilis, which belongs to the large clade having an ACT domain as a carboxy extension, has been extensively characterized [ 30]. 4-Hydroxyphenylpyruvate is an effective competitive inhibitor, as would be consistent with our proposed effects at the catalytic core for a PPA-specific enzyme.
Preferred extensions are subset-maximal admissible sets (equivalently: subset-maximal complete extensions); finally, semi-stable and stage extensions are characterized by maximizing the concept of range.
When G is amenable, these extensions are characterized in terms of piecewise affine maps.
3 val A = ⋀ a ∈ A ¬ val (v a, i ) Now, complete extensions are characterized by the following formula.
The sequence similarity and structural homology of some of these putative effector proteins to C-terminal extensions in characterized evolved VgrG proteins suggest that they may become tagged to the conserved core VgrG proteins which serve as transporters for these effectors, thereby forming new evolved VgrG proteins.
In AAP, the N-terminal extension is characterized by a structurally conserved α1-helix, which is known to affect thermal stability and thermal dependence of the catalytic activity.
In some passages, extension is characterized as a matter of simply preserving or not losing what is given to one at birth (4B12, 4B19, 4B28, 6A10, 6A11), and such passages accord with the water metaphor in suggesting that moral development happens in the absence of abnormal interference.
Furthermore, high-speed high-resolution live imaging revealed that the onset and progression of tail extension were characterized by the presence of dynamic and polarized actin-based protrusive activity at the plasma membrane of individual muscle cells.
A ratio of 3.3:1 of dA to dC insertion opposite M1dG was observed in the extension assays characterized by LC-ESI/MS/MS.
The hydrophilic and highly flexible C-terminal extension, as characterized by solution state nuclear magnetic resonance (NMR) (18), is also essential to efficient chaperone activity, with an important role in solubilizing complexes of the chaperone with misfolded and aggregated species (10).
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