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The observed maximum length of the extension corresponds fully with the extension track length observed in mammals in vivo [29,30].
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Wood Lane station was modified and extended to accommodate the northward extension tracks linking to the E&SBR.
Notably, addition of the single-strand binding protein replication protein A (RPA) results in formation of longer extension tracks (Fig. 1B, lane 7).
We found that human polymerase δ, together with PCNA, efficiently extends D-loops, with the extension tracks reaching up to 2 kb.
Although less efficient compared to Pol δ, Pol η and Pol κ, they extended the D-loop substrate while generating shorter extension tracks.
Indeed, our data suggest that Pol η and Pol κ affect recombination in vivo and can participate in D-loop extensions in vitro that result in generating short extension tracks.
In addition, the presence of PCNA on the D-loop regulates the length of the extension tracks by recruiting various polymerases and might present a regulatory point for the various recombination outcomes.
We further demonstrate that polymerase η extends D-loops independently of PCNA, pointing to the possibility that the presence of PCNA may regulate the length of the extension tracks and thus alter the outcome of HR.
The same lever set-up used in Experiment 1 was moved with shoulder flexion and extension to track an on-screen cursor vertically up the screen (shoulder flexion) or down the screen (shoulder extension).
To operate longer trains, the extension of tracks at sidings, crossing stations and terminals is needed.
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