Sentence examples for extension time to from inspiring English sources

The P. falciparum 18sRNA gene was amplified using methods described by Padley et al [34], modified by shortening the extension time to 50 sec and extending the number of cycles to 45, or as described by Rubio et al [35].

We set the 72°C extension time to 30 seconds for 150 400 bp libraries.

PCR reactions were as above except the annealing temperature dropped to 44°C, extension time to 10 s and 12 cycles employed.

Assays were performed for 1 h, but increasing extension time to 2 h had only a minimal effect on maximal tail length (Supplementary Material, Fig. S4).

Quality control of sample DNA was performed with the fungal-specific primer pair ITS1F/ITS4 (White et al. 1990; Gardes and Bruns 1993) and cycling parameters as above except that the annealing temperature was set to 54 °C, extension time to 45 s, and cycle number to 35.

We replaced the use of Sequenase v.2.0 DNA polymerase (Affymetrix, CA) with Bsu DNA polymerase, large fragment (NEB, MA), and substituted the corresponding Sequenase reaction buffer with NEB Buffer 2. We used 2 U of Bsu DNA polymerase per pre-amplification cycle, extended the pre-amplification extension time to 20 min and used 32 pmol P1 primer.

The first profile stretched all extension times to 6 min.

Cycling extension times were increased to 60 s and final extension times to 10 min for accD and clpP.

Cycling extension times were increased to 90 s and final extension times to 10 min for ycf1-ndhF and rps15-ycf1.

The Act also incorporated another bill of 1893 to grant an extension of time to build the southern extension to Clapham.

What many people don't understand is that filing an extension just puts off the inevitable, because it's not an extension of time to pay, it's just an extension of time to file.