Sentence examples for extension time for from inspiring English sources

Pair it with an Apple device and it can measure your child's pitch speed, ball rotation, spin rate, arm extension, time for the ball to get to home plate, and other pertinent metrics.

The extension time for the iPCR is 3 minutes, while the extension time for the vPCR is adjusted to 14 min. to allow sufficient time for amplification to occur.

The extension time for each cycle was prolonged to 1 min and 30 s when amplifying the 5′-flanking region and exon 7, to compensate for the length of these PCR products.

For PCR, use a touchdown protocol with the annealing temperature stepping down (−1°C/cycle) from 65°C to 55°C, followed by 30 cycles at 55°C, and a 45 s extension time for all cycles.> f.

A touchdown PCR cycling program was used where the annealing temperature was gradually lowered from 70 to 56 °C in 2 °C increments with a 3-min extension time for each cycle.

PCR reaction volumes and cycling conditions were as above, except the extension time for initial long-PCRs to generate the entire locus (∼8 10 kb) was 10 min. PCR products were then Sanger-sequenced as above and complete mating-type loci sequences were deposited in GenBank: KF961042 KF961047.

PCR was performed using Biolase (MidWest Scientific, St . Louis MO) with 1μl of the RT reaction as previously described [ 31, 33], using a 61° annealing temperature and 20 sec denaturing, annealing and extension times for 30 cycles.

Using Eppendorf thermo cycler (Eppendorf AG Hamburg, China), PCR was programmed as, 1 cycle of 4 min at 95°C, 35 cycles of 45 seconds at 94°C, 30 sec at different temperatures (annealing temperatures and extension times for each primer pair are provided in Table  2) and last cycle was followed by a final incubation for 10 min at 72°C.

An 18 kb PCR product was obtained using an extension time of 24 minutes for each cycle but not using 20 minutes extension time (Additional file 3).

Each STR primer pair was optimized for annealing temperature and extension time: 94°C for 3 min., then 60 cycles of 94°C for 20 sec., 54 62°C (-0.1°C/cycle), 72°C for 45 90 sec., and a final extension at 72°C for 5 60 sec.

The extension time required for every cycle in these PCR procedures is more than 3 min to obtain long PCR products, which is very time consuming.