The differential recruitment of these pathways possibly reflects distinct patterns of frequency-dependent facilitation at giant MF synapses, which terminate on pyramidal neurons, whereas small synapses made by filopodia-like extensions terminate on inhibitory interneurons in CA3 (Acsady et al. 1998).
Contig-end extension terminates when (1) the extended sequence overlaps with the other contig end at the other side of a gap, (2) an extended sequence with no overlap with the contig end at the other side of gap is 1000 bp longer than the size of the gap in the reference genome, or (3) no reads can be found to make a new round of extension.
An SPFL statement read: "The SPFL player registration of Heart of Midlothian FC player Danny Wilson would, had he not agreed a contract extension, have terminated on 30 June.
Extension is terminated when a position is encountered that does not meet these user-specified criteria.
A final extension was terminated by a final incubation at 72 °C for 10 min.
Once the extension has terminated in both directions the number of k-mers visited is written to the output file.
Primer extension was terminated after incorporation of dAMP opposite the 3′-Thy by exo-free Klenow fragment and pol IV (dinB) of E. coli, whereas Taq polymerase was completely blocked at the nucleotide preceding the cross-link.
In regions which may exhibit flexibility, such as the H95:H96:H97 three-helix junction, primer extension is terminated on the first guanine of a 3′-GG-5′ pair (G3045 G3044), suggesting a monofunctional adduct or long-range RNA cross-link.
It is interesting that in an earlier in vitro study primer extension was terminated after incorporation of dAMP opposite the 3′-T of G[8,5-Me]T by pol IV. (12) In contrast, the data of our in vivo study showed that pol IV is quite efficient in bypassing the cross-link.
For the G[8,5-Me]T cross-link, primer extension was terminated after incorporation of dAMP opposite the 3′-T by exo-free Klenow fragment and pol IV (dinB) of E. coli, whereas Taq polymerase was completely blocked at the nucleotide preceding the cross-link (18).
