In this study, we showed that this N-terminal extension segment is critical for the pronounced aggregation properties of Mca1.
Importantly, both long Mca1 variants, Mca1454-GFP and Mca1451-GFP, share the 19 amino acids N-terminal extension segment (MSLEVYLNYHQRRPTRFTI, Figure 1) and show very similar aggregation properties in vivo.
A single fluorescence measurement was conducted at the end of the 72°C extension segment.
The conditions of amplification consisted of an initial denaturation step at 95°C for 5 min as a "hot start" followed by 40 cycles at 95°C for 30 s/60°C for 30 s with a single fluorescence detection point at the end of the relevant annealing or extension segment.
Small K-values for these two models indicate that the up-dip extensions of segments B and C have little effect on damage done to sites throughout the Kinki region.
The principles are explained based on a three segment design but extension to more segments is straightforward.
Therefore, all protein extensions and rRNA expansion segments (ESs) are annotated on the basis of comparison with human ribosomes.
The docking procedure consisted of rigid body docking of terminal core fragments, central loop closure, constrained all-atom refinement of the core segment, and extension of the core segment.
The extension of the segments across the gut (Figure 3A, C) and the presence of darker areas delimiting each segment indicate that these latter structures are unlikely to be internal mesenteries but are integral to the segments.
The westward extension of rupture segments with about 10-m slip is indispensable for explaining the historical damage along the Pacific coastal region of Kyushu, which is similar to the findings of Furumura et al. (2011).
