Sentence examples for extension probe from inspiring English sources

Panel customization was accomplished via specific amplification primer and extension probe design.

Primer extension was then carried out by combining 2 µL of the purified PCR product with 5 µL of the ABI Prism SNaPshot Multiplex Kit Applied Biosystemss, CA, USA), 2.5 µM of the appropriate extension probe, and 3 µL of water, in a total volume of 10 µL.

Unincorporated dNTPs were deactivated using 0.3 units of shrimp alkaline phosphatase at 37 °C for 40 min and primer extension was carried out using 7 14  μℳ of each primer extension probe (depending on the mass), 1 unit of iPLEX termination mix, and 1 unit of iPLEX enzyme.

Unincorporated dNTPs were deactivated using 0.3 units of shrimp alkaline phosphatase (Clontech Laboratories, Inc., Mountain View, USA) at 37° for 40 min and primer extension was carried out using 7 14  μM of each primer extension probe (depending on the mass), 1 unit of iPLEX termination mix, and 1 unit of iPLEX enzyme.

The assay group file containing the PCR primers and the iPLEX extension probes can be supplied on request to the corresponding author.

PCR primers and primer extension probes were designed using SpectroDESIGNER software (Sequenom), and all PCR amplifications and primer extension reactions were generated by PCR-ABI 9700 thermocyclers (Applied Biosystems, Foster City, CA, USA).

All PCR primers and primer extension probes were designed using Primer 3 [ 45] and Array Designer2 (Premier Biosoft) software.

To decrease the number of multiplex PCR tubes, manual modification of some PCR primers and extension probes was conducted.

aSequences are 5' amplification primers, 3' amplification primers, or primer extension probes, and are preceded by F-, R-, and E- respectively.

The primer extension probes were designed with varying lengths of deoxythymidine monophosphate homopolymers ranging from 30 to 76 bases to allow for discrimination of the products by size.

The polymerase chain reaction primers and extension probes that were used to type met1 region loci are presented in supplementary table S1, Supplementary Material online.