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The default gap opening and gap extension penalties were used.
The default gap opening and gap extension penalties were used (15.0 and 6.66).
Pairwise and multiple alignments were performed using BLOSUM protein weight series matrices, with default gap opening and gap extension penalties.
I rewarded matches and penalized mismatches with one point each and varied the gap opening and extension penalties to identify the best alignment conditions.
Analyses were based on the 'SCOP40 iter5' database, gap opening and extension penalties of 10 and 1, respectively, and the BLOSUM62 substitution matrix.
I used combinations of gap opening penalties of 5, 4, 3, 2, and 1 with gap extension penalties of 2 and 1.
Without such a consideration, the alignment is more sensitive to user-supplied parameters such as match and mismatch scores, and gap opening and extension penalties.
With small modification, SlideSort can deal with gap opening and gap extension penalties.
Different gap opening and gap extension penalties lead to different Indel numbers and sizes.
We used gap opening and gap extension penalties of 2.4 and 0.15, respectively.
The default gap open and extension penalties were used for BLOSUM62 while for BLOSUM90 and SMAT80, 10 and 1 were gap open and extension penalties respectively (best parameters for matrices which have entropies similar to BLOSUM90).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com