Sentence examples for extension of the upstream from inspiring English sources

After processing, the 2A peptide remains as a C-terminal extension of the upstream protein and all products downstream of 2A contain a proline residue at the N-terminal (Ryan et al. 1991; Donnelly et al. 2001b; De Felipe et al. 2003).

iii) 3'-end extension of the upstream gene due to either a frameshift at 3'-end of gene or point mutation at the stop codon.

In heterologous (artificial polyprotein) contexts, the 2A sequence remains as a C-terminal extension of the upstream gene: potentially a consideration when using longer, albeit more efficient, F2A sequences.

However, it should be noted that (i) 2A remains as a C-terminal extension of the upstream product, and (ii) proline forms the N terminus of the downstream protein.

Firstly, whilst the longest F2A sequence (58aa) tested to date was shown to produce the most efficient cleavage, the C-terminal F2A extension of the upstream translation product may have an effect on protein confirmation and activity - such as in the production of monoclonal antibodies or expression of enzymes.

The longer versions of 2A described below are therefore 2A plus N-terminal extensions of the upstream capsid protein 1D, but for simplicity referred to as F2A [ 8– 11].

In most cases, the new gene ends did not correspond to 3′UTR extensions of the upstream transcripts but to novel large intervening non-coding RNAs (lincRNAs).

In the case of SPN23F16220, the transcription follows the direction expected from the annotation, with the BOX element forming a 3' extension to the upstream three CDS operon, as confirmed by RT-PCR.

A slight modification of this approach addresses FDM at the beginning or end of the gene model and enables extension of the sequence upstream or downstream or the original gene model termini (Figure 1, D).

Thus, if selection is responsible for the removal or extension of short upstream regions, it may simply be that selection has not had enough time to act in these very young sets of inter-CDS regions (although in this case we might expect similar patterns in the sibling species).

A putative translation indicates that an upstream extension of the 61-nt exon into and beyond the 193-nt would lead to a continuous extension of Cpeb3 protein beyond the N-terminus.