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Using PAS, the rating was set for intensity and extension of staining, ranging from 0 (no staining) to 3 (intense and extensive staining), respecting tissue specificity scoring when adequate.
This pattern was also seen in the tg samples, with a noticeable increase in the amount of staining, as well as an extension of staining to varicosities along the axonal projections through the outer granular layer.
The extension of staining in each cancer specimen (i.e., the percentage of tumor cells displaying any degree of staining) was rated as absent (no stained cells); limited (≤ 35% cells); moderate (36%69%%); or extensive (70%100%%).
The differences observed between our study and others, besides the use of different antibodies, might be also due to the diverse immunohistochemical assessment, while in the present study we considered both intensity and percentage of immunoreactive cells, other authors considered only either intensity or extension of staining [ 14, 22- 25].
For the EGFR staining, three of the authors (GT, CGC and LMM) scored the specimens as positive, when any intensity and extension of staining was apparent, or negative when no signs of staining at all were seen, as described by other authors (Albanell et al, 2001).
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Combined assessing of staining intensity and extension was used to evaluate GCRG213p expression.
The extension of the staining was scored as the percentage of positive cells (0 100%), and the intensity of the staining was assessed using a 4-value scoring system (0, below the level of detection; 1, weak; 2, moderate; and 3, strong).
The atnup62 mutation resulted in an extension of the staining to the external upper part of the hypocotyl, where AtNUP62 is expressed (Fig. 3d, right).
e Photograph of stained tissue.
Many of these have been based upon an extension of Annexin V staining in vitro, which indicates apoptotic cells through binding to phosphotidyl serines [ 69].
Briefly, each tissue spot was digitalised to the system software, and a computer algorithm based on the intensity and extension of the brown staining attributed numerical values to each spot.
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