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In fact, one often encounters a situation that extension of complementary strands by DNA polymerase is halted in an inverted repeat region in reactions for DNA sequencing.
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Site-specific mutations were inserted in primer sequences in order to prevent extension of reciprocally complementary primers.
To address if 5′-phosphate (5′-P) binding by each monomer is an absolute requirement for in trans extension of short complementary ends, we used a set of 3′-protruding molecules possessing either none or one 5′-P at only one of the two DNA ends.
Accurate DNA replication is therefore governed by correct nucleotide insertion, and in the case of polymerases with proofreading ability, by the favored extension of correctly paired complementary strands[ 5].
Rather than trying to distinguish between systems medicine and other disciplines, our review is based on the premise that systems medicine is a natural extension of, or is complementary to, current models for clinical decision-making.
These propositions should be regarded as input for discussion and discourse: 1. 'Arts as a manifestation of knowledge': By defining arts as a manifestation of knowledge (in complementary extension of a more traditional understanding of the aesthetic dimension of arts), the artistic research and arts-based innovation then interflow directly with art and arts.
Long-term investments in agricultural extension and reductions in the costs of complementary inputs are critical for the diffusion of MTM.
Primer extension analysis and 5′-rapid amplification of complementary DNA ends studies show that there is a single transcription start site 232 nt upstream of the translational start codon.
Truncated mCitrine (i.e. residues 1 to 232) was fused to mKate2 by overlap-extension PCR using the following set of complementary primers: 5'-GCCGGGATCACTCTCATGGTGAGCGAGCTGATTAAG-3' and 5'-CTTAATCAGCTCGCTCA CCATGAGAGTGATCCCGGC-3'. Mermaid was kindly provided by Dr. Miyawaki (RIKEN BSI, Japan).
"It's pretty complementary; an extension of what QuickBooks already does".
Following the basic idea of CAN-based algorithms, we propose an extension of the CAN algorithm to complementary sets of sequences (which we call CANARY).
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