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On the other hand, substitution of a less selective inosine nucleotide did not convincingly improve the extension efficiency.
The patella works as a lever arm by separating the point of action of the knee extension system from the rotation centre to increase the extension efficiency [1].
At the high salt concentration (150 mM KCl, lanes 13 24), both polymerases showed similar PCNA-dependent D-loop extension efficiency (Fig. 4C and D).
Gel permeation chromatography (GPC) in dimethylformamide (DMF) indicated a relatively high chain extension efficiency and a low copolymer polydispersity (Mw/ Mn<1.20).
Unlike the control dA-extension reactions, the bypass extension efficiency of the control dG nongapped DNA could not attain the same levels as that for the reactions carried out on the gapped substrate.
The FABP in the G*C A series at n + 3 adopted exclusively the B-conformation, yet exhibited a 6-fold greater insertion extension efficiency of dATP than G*C T for the matched series.
Similar(45)
Due to the enzymatic activity, single MMs present at the last 3 4 positions from the primer 3′ end exhibited zero or minimal (< 3.9%) extension efficiencies.
In this study, a modified single base extension assay was used to systematically compare the extension efficiencies between a perfect-matched (PM) primer and its single-MM primers with all possible MM types.
It was further shown that the extension efficiencies of these MM types could be improved with a factor of 3.25 on average in relation to the decrease in the annealing temperature by 7 °C.
The extension efficiencies of single-MM primers, which were generally lower or equivalent to that of the PM primer, were observed to strongly depend on the MM location and/or type.
In the past, the possibility of different extension efficiencies was discussed as a mechanism for the increase of the mutation rates of 5mC in vivo.
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