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We also corrected several start sites with the help of our original N-terminal extension database.
Among them, three annotated ORFs were extended upstream through comprehensive analysis against an in-house N-terminal extension database.
As a result, 1311 peptides were collected in the customized extension database (Additional file 3, Table S2).
All MS-derived peptides were screened against both the S. flexneri 2a str.301 protein database (downloaded from NCBI) and the customized N-terminal extension database (see Methods section).
To detect potential extended TSSs of the predicted coding sequences, we constructed a specialized N-terminal extension database, using a similar strategy as previously described [ 16] with some changes.
The peptide from the new start codon to the 33rd amino acid residue downstream of original start site was collected into the extension database, except for those CDSs whose start codon was the same as the previous annotation.
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Errors of this kind may be ameliorated by extension of the reference database.
These extensions implement database objects such as new data types, application-level object classes, analytic algorithms, and external data-access facilities.
The Proteios system, was initially developed as an open source system for storage, organization, analysis and annotation of Proteomics experiments and is now part of the ProSE, an analysis platform targeted for multiple users that integrates management and analysis of the data with a programming interface that enables local extensions and database access [ 13, 14].
Jaspa (Java Spatial) is a novel spatial extension for relational database management systems (RDBMSs).
We extend this model by transplanting a complete scriptable chemical information processing toolkit into a database extension.
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