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As in T. castaneum, the cecropin possesses an atypical tyrosine-rich c-terminal extension (data not shown).
In addition, transcriptional starts within mca and pup fragments and the respective sequence motifs resembling SigA-dependent promoters were localized upstream of these genes by primer extension (data not shown).
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As a result, MpSIG1 was confirmed to be composed of 574 residues because 5′-RACE did not detect additional N-terminal extensions (data not shown).
However, in the 299 bp IR between tuf and lon, a promoter-like sequence was found by primer extension analysis (data not shown).
17993534|This finding was confirmed by primer extension analysis (data not shown) and by recently deposited microarray data in the GEO database (http://www.ncbi.nlm.nih.gov/geo/ ; accession number GSE8478) Deposition: If the evidence statement shows the data were deposited.
17993534|This finding was confirmed by primer extension analysis (data not shown) and by recently deposited microarray data in the GEO database (http://www.ncbi.nlm.nih.gov/geo/; accession number GSE8478) Figure 3 shows the overlap between GEO (in orange) and GEO curation in MEDLINE (SI) (in red), whereas Figure 4 shows the overlap between PDB (in blue) and PDB curation in MEDLINE (SI) (in red).
The specific PCR product is rarely detectable in this mix even after P-extension step (data not shown).
Structural data (NMR and X-ray crystallography, Matthew Preimesberger and Selena Rice, personal communication) also concur that the N-terminal extension is disordered (data not shown).
We found no difference between the responses of wild type and DILP mutant flies to the addition of EAA to DR food, indicating that IIS is not required for the lifespan extension by DR (data not shown).
Resultant morphological defects in survivors of Chp MO1/2 at 24 hpf included shortened antero-posterior (A-P) axes, failure in yolk extension and rounded somites (data not shown).
To optimize the multiplex amplification, the concentration of MgCl2 and the annealing and extension temperatures were varied (data not shown).
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