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These parameters provide fine criteria for estimating convergent extension cell movements during gastrulation.
Ptpre plays a role in osteoclast bone adhesion and resorption in mammals and in convergence and extension cell movements during zebrafish gastrulation [19], [34].
Moreover, either over-expression or knock-down of Hipk1 leads to perturbed convergent extension cell movements involved in both gastrulation and neural tube closure.
Previously, we have shown that Fyn and Yes operate in a pathway that converges with non-canonical Wnt signalling to regulate convergent extension cell movements during vertebrate gastrulation (Fig. 2 a d)[13].
Nevertheless, during gastrulation Hipk1 is necessary for transcriptional activation of a subset of Wnt-responsive genes in the involuting mesoderm, and disruption of Hipk1 by either over-expression or knock-down leads to severe defects in convergent extension cell movements.
We further demonstrated that the loss of cfl1 induced by its respective morpholino results in the interference of epiboly, convergent extension, cell protrusions and directed cell migration during gastrulation.
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Our data indicate that plant responses to external stimuli are strongly organ-dependent and underscore the need for a more thorough survey of organ-specific and, by extension, cell-specific responses in Arabidopsis and other plants [ 3].
By cell membrane labeling and ratiometric analysis of cell shape and orientation, we found that cell polarization is reduced upon N-Ras-I24N expression (supplementary material Fig. S3), which is consistent with the observed convergence and extension cell-movement defects.
This data indicate that plant responses to external stimuli are strongly organ-dependent and underscore the need for a more thorough survey of organ-specific and, by extension, cell-specific responses in Arabidopsis and other plants [ 3].
Quantification of convergence and extension cell-movement defects by analysis of the ratio of the width of krox20 staining and the length from somite-1 to somite-8 was done using ImageJ software as described before (van Eekelen et al., 2010).
Thus, we set out to examine the effects of Ptenb on gastrulation cell movements and found that knockdown of ptenb by MO cell non-autonomously disturbs epiboly and convergent-extension cell movements during gastrulation in zebrafish.
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