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The multiplex primer iPLEX extension assay was performed as previously described23.
A panel of 92 inflammatory proteins were analysed simultaneously in the supernatants using the proximity extension assay on the Proseek Multiplex inflammation panel (Olink Bioscience, Uppsala, Sweden).
A non-specific competitor ssDNA derived from the 15 nt of spacer DNA of the telomerase extension assay substrates with sequence 5′-CTAGACCTGTCATCA-3′ was added where indicated.
An in vitro primer extension assay indicates that three out of five compounds are effective HIV-RT inhibitors.
The primer extension assay can also be used to map DNA strand breakage generated by other footprinting methods, and to determine DNA sequence directly from the yeast genome.
We have developed an improved method for photofootprinting in vivo which utilizes the thermostable DNA polymerase from T. aquaticus (Taq) in a primer extension assay.
The PC12 cell neurite extension assay confirmed that the presently developed multi-tubule conduit-filler constructs were reliable for effectively preserving the bioactivity of released NGF.
The primer extension assay was performed using a standard procedure described previously [37].
Compound hits were validated in a primer extension assay (Figure 4).
The single base extension assay conditions, multiplex details and primer sequences were as previously described in detail [1].
We first identified the transcription start site (TSS) of the human Prep1 gene using a primer extension assay (see Methods).
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