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Mid-stage clusters M1 and M2 are generally associated with many of the same genes in the early clusters but also include the later processes of 'axon extension' (Additional file 10: Figure S10), in agreement with the extensive neuronal differentiation and synapse formation that occurs at P7 [ 48].
Therefore, by logical extension, additional gains in physical activity will result in greater incremental energy expenditure in a human than in a rodent.
By extension, additional copies of SLC2A3 may confer protection to patients, and lead to HD manifesting later in life.
In each case, the signal peptide (Additional File 5) immediately precedes the mature enzyme region which, in turn, is followed by a C-terminal extension (Additional File 6).
These polypeptides also contained a signal peptide (Additional File 5), the mature enzyme region and a C-terminal extension (Additional File 6) but they too lacked a propart (Table 3 and Additional File 5), just like PxAP6 and PxAP7.
When the progression temporarily stops, the green coronin label becomes dominant at the very rim (28 second frame), to be replaced by the actin label as soon as the cup resumes extension (Additional file 4).
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Bet3p is very highly conserved in terms of length throughout the eukaryotes, whilst Bet5p in Arabidopsis thaliana and Plasmodium falciparum contains a short N-terminal extension as compared to the S. cerevisiae and H. sapiens orthologues, and Caenorhabditis elegans has two isoforms, both of which have extensive C-terminal extensions (additional file 2).
For 109 of these proteins we have detected N-terminal peptides covering the predicted extensions (Additional file 10: Table S9).
The PxAP10 - 12 polypeptides contrast with their PxAP6 - 9 counterparts in having the archetypal AP architecture of a signal peptide followed by a propart (Additional File 5), a mature enzyme region, terminating with C-terminal extensions (Additional File 6).
This intriguing possibility is rendered all the more fascinating with the realisation that, in contrast to the PAGs/Bla g2, these Phytophthora orthologues are likely to be membrane-bound through their C-terminal extensions (Additional File 6) and might function as a novel type of surface receptor.
Finally, a third line of extension adds additional querying capabilities, such as range queries [18, 19], by combining additional routing links, and well-chosen hash functions [20].
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