Some partial sequences were extended to full length using 5' and/or 3' RACE.
Several partial cDNAs of the P450s and UGTs were extended to full length by 5' and/or 3' rapid amplification of cDNA ends (RACE).
The P450 sequences ZfCYP4G47, ZfCYP4G48, ZfCYP4L17, ZfCYP6AE27, ZfCYP6CT1, CYP9A36, ZfCYP9A37, ZfCYP304F2, ZfCYP332A3, ZfCYP333B8, ZfCYP379A2, and ZfCYP379A3 were verified by Race-PCR using the SMART RACE cDNA Amplification Kit from Clontech (cat. 634914), and partial sequences were extended to full length.
In fact, this property extends to full-length receptors; some pLGICs in invertebrates are activated by acetylcholine as well as serotonin [ 6].
This observation was recently extended to full-length LmrA in proteoliposomes [31].
At the highest enzyme concentrations used (32 nM) 72% of control templates were extended to full-length product.
As previously observed in the running start primer extension assays, these 22-mer products were also not extended to full-length products by the action of hRev1 alone.
Since all of the genes encode relatively long proteins, most of the collection comprises gene fragments which cannot yet be extended to full-length.
The M1dG lesion blocked incorporation of the initial nucleotide opposite the lesion, but 14% of templates were extended to full-length product.
At the same time, the detected gene fragments might be extended to full-length genes with further improvement of the genome assembly.
The leading strand is continuously extended to full-length product; consequently, once Pol α has switched to the DNA elongation mode, it does not appear to switch back to the priming mode.
