Sentence examples for extending step from inspiring English sources

In the extending step, the intermediate genes on the k-shortest paths between the DE genes were added to form the final responsive gene modules.

The following temperature profile was used: an initial denaturing step at 94°C for 1 min; 45 cycles of denaturing at 98°C for 10 s, annealing at 57°C for 30 s, and extending at 68°C for 1 min per Kb; and a final extending step at 68°C for 10 min.

The following general profile was used: an initial denaturing step at 94°C for 2 min; 35 to 40 cycles of denaturing at 94°C for 20 s, annealing at 42 55°C for 20 s, and extending at 68°C for 60 s per kb; and a final extending step at 68°C for 7 min. PCR amplicons were purified by ethanol precipitation.

The following temperature profile was used: an initial denaturing step at 98°C for 2 min; 35 cycles of denaturing at 98°C for 10 s, annealing at 50°C for 30 s, and extending at 72°C for 1 min; and a final extending step at 72°C for 1 min.

The PCR program consisted of an initial denaturing step at 94°C for 5 min, 35 amplification cycles, and an additional extending step at 72°C for 3 min. For the primer pairs FF1/FR1, ITS5/NL4, Fulys2-F03mix/Fulys2-R01, and Fulys2-F04mix/Fulys2-R04mix, the amplification cycles were 94°C for 30 s, 52°C for 40 s, and 72°C for 1 min and 10 s.

Presented here are results of this search, along with 21 potential intron retaining or exon extending STEPs where allele population frequency and EST data has shown a disproportionate retention of one STEP allele, as well as a spliced form.

ISO 14649 is strictly harmonized with ISO 10303 (STEP), hence the name STEP-NC meaning an extended STEP for numerical control.

Then, the existing RA [11] has been extended (step 4) and evaluated (step 6) based on new empirical data gathered from the implemented architecture (step 3).

However, it is seemingly impractical to start with the most fundamental structure of the system, and then to extend step by step to obtain its holistic behaviors.

Based on incremental design and implementation of the prototype system (step 3 of the research method [40]), the metadata management layer has been extended (step 4) and evaluated (step 6) in this work.

This paper presents a new approach to further extend STEP's capability for the simultaneous energy targeting, optimal placement of process utility systems that include flue gas streams with variable-temperatures and flowrates, and design of heat recovery networks featuring such targeted utilities.