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The primers were extended (underlined sequence) to allow digestion of the amplified product with NdeI and BamHI and it was then ligated into plasmid pET15b (Novagen: San Diego, CA).
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This extends and underlines the knowledge of the effect of PIK3CA mutations and PI3K pathway activation on HER2-inhibitor treatment response observed on patient breast tumour samples.
In addition, by performing oxidative refolding studies on representative cyclotides the knowledge of their in vitro oxidative folding behaviour was extended and this underlines the high dependency of folding yield to their inter-cysteine loop sequences and careful choice of the folding conditions.
The effects of pretubulysin in growth inhibition seen in vitro are underlined and extended by an in vivo chicken chorioallantoic membrane (CAM) model, showing that 10 nM pretubulysin significantly inhibited tumor growth of T24 cells after implantation onto the chorioallantoic membrane of chicken embryos.
However, extended periods for endocrine treatments underline the importance of investigating side-effects.
In order to underline its applicability, we extended an existing IAM tool proposed in [6] with DPMP functionality.
We were able to extend the VDAC and Sam50/Tob55 sequences (underlined letters in fig. 1) but not the Tom40 sequence.
Moreover, after a thorough analysis of the most recent results available in the literature they underlined the need to extend this kind of investigation to a larger number and a wider range of specimens [ 36].
All that was noted at first was a yellow card for Henderson after a foul on Yoann Gourcuff, but France then underlined their continuing keenness by extending the lead.
Union opposition to driver-only operation of doors, a practice GTR is extending, is a stated safety concern, but underlined by the fear that job cuts will inevitably eventually follow.
These data confirm and extend previous data underlining the central role of the determination of ANAs in defining the diagnosis, subset allocation and prognosis of SSc patients.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com