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Perhaps in reaction to this fast expansion of what constituted an education, the next several decades practiced an extended purification rite.
The extended purification by cation exchange chromatography using a Ca 2+-charged chelating Sepharose (GE Healthcare) was essential for the successful crystallization.
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We previously speculated that IMAC could be extended from protein purification to the purification of nucleic acids, by separating nucleotides and single-stranded DNA/RNA from double-stranded DNA.
Since this purification strategy is efficient, scalable while non-destructive, we hope it could also be extended to the purification of other anisotropic nanoparticle systems not limited to gold.
An extended scheme of purification was followed as described in the methodsection to purify the nuclease from hepatopancreas, a digestive gland of shrimps.
As a future perspective, isolated magnetic organelles can be extended to protein/protein complex purification for biochemical and structural biology studies.
PCR reactions using iPLEX consumables, inactivation by shrimp alkaline phosphatase, extended PCRs, ion exchange purification and spotting onto each SpectroChip II matrix were performed according to the manufacturer's protocols.
For modifications and details of the Lis1 and Nudel purifications, see Extended Experimental Procedures.
It extends for the purification of polluted rivers and streams and also meeting the requirement on the standards of wastewater effluents without building massive structures as compared to the other conventional methods as needed in wetland construction [ 16].
A specially designed forward primer containing extended polyA20/5Sp9 facilitated gel electrophoresis purification of ssDNA after PCR amplification.
Mitochondrial transplantation provides an efficacious cardioprotective protocol that can be used either as an exclusive intervention to ameliorate myonecrosis and enhance myocardial function or could be used as a primary intervention prior to subsequent auto-, allor or xeno-geneic cellular regenerative interventions requiring extended times for cell isolation, purification and expansion.
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