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Stable sand propylene glycol nanofluids were formulated by dispersing sand nanoparticles (20 25 nm) produced by stirred bead milling, in propylene glycol through extended probe ultrasonication.
However, since the false positive rate will be higher in this set of probe sets due to more noisy expression data, we chose not to include these in our list of putatively extended probe sets.
The multiplex SNaPshot detection of single-base extended probe primers was based on fluorescence and extended length detected by capillary electrophoresis on an ABI3130XL Sequencer (Applied Biosystems).
Its first known exon was expressed in the colon but not the thymus, whereas in MG-thymoma an extended probe set located just before the first exon was highly expressed (Figure 3K), indicating alternative splicing in this large (0.2 Mb) gene.
The extended probe set targets the region in between, which is a highly conserved region.
Methylation scores were calculated for each extended probe site using default values.
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Probes targeting exons with EST evidence are referred to as "extended probes".
To explore the broadest range of transcripts, Affymetrix extended probes were imported, yielding transcripts of which 112,207 have annotation with 50,601 at the gene or mRNA-level.
Our results show, that the overall number of phylogenetically redundant probes available could be more than doubled by extending probe design to the 23S rRNA.
In addition, the possibility to generate single-strand probes in the range of 150 500 nucleotides by a sodium hydroxide treatment of immobilised probes with subsequent elution of the remaining biotinylated strand, opens up for new microarray applications that would extend probe length beyond current oligonucleotide synthesis limits.
Only 48 of the 420 probes from the extended SLA probe set and 41 of the 73 from the immune probe set are present on both microarrays.
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