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In contrast, CF caused extended lag phase and reduced cell yield of strain DCB-2 in a dose-dependent manner in the presence of fumarate or 3-Cl-4-OHPA (Figure 1B).
In spite of efficient sulphate reduction performances in the first halves, all the isolates showed extended lag phases showing negligible growth in the initial 10 days of anaerobic incubation periods (Fig. 2).
In liquid minimal medium M9 with glucose as the sole carbon source at 37°C, the mutant strain had extended lag phase when compared to the parent strain.
As the total polymer weight in floating pulsatile layer of tablet was replaced with 100% of polyox WSR205, it had shown extended lag period of 8 ± 0.5 h which is higher than the predetermined lag time.
The intervening period of comparatively low net diversification rates reflects the often extended lag times between the origins of the different families and the basal radiations within the crown groups, and could possibly be the signature of the pattern of competitive subclade replacement known for at least canids and felids.
Because 100 μM CF significantly inhibited the growth rate of strain JW/IU-1 and caused the extended lag phase and the reduced cell yield of strain DCB-2 in medium containing 3-Cl-4-OHPA (Figure 1), we investigated the effect of CF on dechlorination of 3-Cl-4-OHPA by resting cells prepared from strains JW/IU-1 and DCB-2 cultivated with or without 100 μM CF (Figure 2).
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When HMF was tested combined with other compounds, which showed no effect individually, like levulinic acid, 2-furoic acid and pantoyllacton, inhibition took place, resulting in extended lag-phase and decreased growth rate (Table 8).
These compounds showed no inhibitory effect individually at 0.5 g/l, but when added together with HMF, they extended lag-phase as well as reduced growth rate (Table 8).
ME affected growth parameters, generally extending lag phase length and reducing maximum growth, sometimes with interactive effects with pH.
As Pol ε is titrated into the reaction, Okazaki fragment synthesis is inhibited, confirming that Pol ε suppresses Pol α polymerase but is unable to extend lagging strand primers (see quantification in Figure 5A, right panel).
For monophenolase activity, both chlorocinnamic acids could extended the lag time and decrease the steady-state activities, 2-chlorcinnamic acid extended the lag time just by 5%, and 2,4-dichlorcinnamic acid extended the lag time more than by 30.4%.
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