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In a few studies, extended differentiation times resulted in increased hepatocyte-specific transcript and protein levels suggesting further maturation in vitro.
After the smoothing, an extended differentiation is performed on the column intensity values, which calculates the difference of values having a distance of 15 pixels.
Only with an extended differentiation time are hematopoietic progenitors capable of maturing further, and therefore acquiring a broader developmental potential.
Furthermore, variable amounts of tryptase were observed in the mature human MCs, similar to what was seen in AZIN2, suggesting that extended differentiation in vitro induces heterogeneity in the MCs.
Therefore we assessed TE differentiation in wild type, Mbd3 −/− ES cells and Mbd3b-rescued Mbd3 −/− ES cells subjected to an extended differentiation protocol.
Compared to the work of Jonhsen et al., we decided to use rapamycin in combination with hydroxyapatite scaffolds, thus allowing a more extended differentiation of the cells in culture.
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This indicated the presence of a mesoderm-like cell state characterized by the expression of T. To determine whether differentiation for 2 days would be sufficient for maximizing the induction, we extended the differentiation period to day 7 and then analyzed the gene expression dynamics (Fig 1B).
Then, considering the disciplinary coupling between the dynamic model and the lubrication models, the stiff and nonlinear tribodynamic model is solved with the modified extended backward differentiation formulae (MEBDF).
In this study, a modified extended backward differentiation formulae (ME BDF) scheme is adapted for the solution of nonlinear diffusion equations, with a special focus on the atmospheric boundary layer diffusion process.
We have extended these differentiations beyond day 14 and have not seen any differentiation towards cardiomyocyte or hematopoietic lineages.
Extending the differentiation for 4 days expanded the CD45+ cells which represented 35% of the floating cells (Fig. 2A).
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