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The extended candidate gene set includes genes sharing the same biological pathways with the seed genes, according to the Kyoto Encyclopedia of Genes and Genomes (KEGG, http://www.genome.ad.jp/kegg/pathway.html) and BioCarta (http://www.biocarta.com/) pathway databases.
These modes are added, if they are not already included, to form an extended candidate set ψ M [30].
The SNPs to be prioritized are then assigned putative disease risk scores based on whether they are in the extended candidate gene set and other genomic information of SNPs themselves via a three-step scoring protocol.
Extended candidate miRNA sequences were folded using the standalone version of RNAshapes, which generates multiple folds for each sequence, ranking them by MFE.
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In summary, our results question the assertion of the 'sparse' nature of genetic networks, while validating and extending candidate gene approaches in the analyses of complex traits.
As a first effort to systematically collect and extend candidate IQ-associated genes, IQdb is also useful to better clarify the molecular mechanisms related to human intelligence.
To validate and extend candidate sequences total RNA was purified using the Poly(A Purist MAG Kit (Ambion, Life Technologies, Grand Island, USA).
GSNAP is a splice-mapper that uses a seed-and-extend method that maps part of the gene as substrings, and then uses algorithms to extend candidate matches and locate potential splice sites [ 58].
This extraction is exhaustive, by iteratively extending candidate strings of size 1... k - 1, letter by letter of the DNA alphabet, and checking in each step if the extended string is still overrepresented in the sequence-set.
The Basic Law of Government changed the process used to select the heir to the throne by extending candidates to the grandchildren of Ibn Saʿūd, enshrined the king's right to choose his heir, established a right to privacy, and prohibited infringements of human rights without just cause.
Alignments are extended at candidate loci, requiring that the read has a consecutive stretch of 14 nucleotides exactly matching the reference sequence.
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