The overlapping products from the FP reaction and RP reaction were combined in equal amounts and extended by five cycles of denaturing, annealing, and extending in a solution containing 1× FailSafe PreMix G (PICENTRE Biotechnologies), DMSO (4%), and Platinum Pfx DNA polymerase (0.025 unit/μL).
These starting clones were extended by repeated cycles of searching to obtain neighboring, overlapping clones until the complete coding sequence of midasin was included.
An Islamic Jihad spokesman, Nafez Nazzam, said his group "has no intention to extend a cycle of confrontation with any nation".
The basic formula multiplies emission factors by the number of farm animals, but the model is extended by a factor which considers provisionally the influence of production cycles of various types of livestock on the estimated emissions.
The first cycle was preceded by 7½ min denaturation and the last cycle was extended by a 7 min elongation step at 72°C.
However, as these models are developed to describe the stochastic behaviour of electricity prices, they are extended by a separate data treatment for the deterministic components (trend, daily, weekly and annual cycles) of electricity spot prices.
But I maintain that a cycle of self-love can be perpetuated by a cycle of self-care.
The frequency range of the instrument is extended by seven hundred cycles a second making it easirer to distinguish between consonants that were often confused.
An existing logical model of the yeast cell cycle was greatly extended by adding a further 30 cell-cycle-related genes that we had previously shown to exert a high degree of control over yeast growth rate.
