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Low Z′ factors, narrow dynamic ranges, and/or extended assay times are commonly reported in current gametocyte cell-based assays measuring gametocyte-expressed fluorescent or luciferase reporters, endogenous ATP levels, activity of gametocyte enzymes, or redox-dependent dye fluorescence.
Low Z′ factors, narrow dynamic ranges, and/or extended assay times are commonly reported in current gametocyte assays measuring gametocyte-expressed fluorescent or luciferase reporters, endogenous ATP levels, activity of gametocyte enzymes, or redox-dependent dye fluorescence.
The extended assay period allowed for identification of inhibitors that were degraded.
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In relation to operational issues, LRE provides several attributes that facilitate large-scale absolute quantification, with the potential to extend assay performance to include the general concept of assay robustness.
Megascript kit (Ambion) and poly dT) 3' primer with a 3-fold degenerate (A/G/C) 3' end (for site of mRNA cleavage assay), poly dT) 3' primer (for PAT assay), or random hexamers (for extended transcript assay) were used to prepare cDNA.
In order to ensure that the effect observed in the glass test tubes was present on other abiotic surfaces and not specific for strain NCTC 11168, we repeated the previous assay using polystyrene plates and stainless steel coupons and extended the assay to three other C. jejuni reference isolates (81116, 81-176, and RM1221) and one C. coli clinical isolate (15-537360).
We developed a tissue assay of PTEN function to test the significance of its protein phosphatase activity and extended this assay to discover non-catalytic functions of PTEN.
The first DASL assay was limited to 1536 probes targeting 502 cancer related genes [8] [10], but Illumina has extended the assay to include 24526 well-annotated transcripts.
This new sensing strategy could be easily extended to assay other proteases by simply changing the peptide substrate.
This new methodology can be easily extended to assay other biomolecules by simply changing the recognition sequence with the substrate aptamer.
In this study, we extended the assay to include all 24K well-annotated RefSeq genes.
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