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Western blot analysis of 22 DAP W64A + and W64A o2 maize endosperm was performed to extend the transcript profiling and proteomic analysis; quantitative measurement by densitometry is shown in Table 2.
Here, we used the Medicago GeneChip® to extend the transcript dataset for mycorrhizal roots and coupled this with LM and RT-PCR to assess gene expression specifically at the cortical cell-arbuscule interface.
In the present study we focus on further analysis of transcript biomarkers in predicting response to RTX in order to determine the value of the proclaimed transcript markers and extend the transcript profile associated with response outcome.
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ASPic will predict them correctly, while ASPic-GeneID may try to extend the transcripts with additional predicted exons.
Other studies showed that asRNA can also hinder RNA polymerase extending the transcript encoded in the opposite strand by transcription interference (reviewed in [ 68]) or can affect translation of the target gene by regulation of ribosome binding [ 69].
Since our previous work showed upregulation of FABP7 transcripts in the prefrontal cortex of schizophrenic postmortem brains (26), we extended the transcript expression studies to FABP3 and FABP5 using the same sample set.
Since for most species in Ensembl full length cDNA sequences are limited, extending the transcript length using a species-specific UTR size is a consistent means to successfully map probe sets to Ensembl transcripts.
Various RNA samples, as shown in Table 1, might extend the variation of transcripts in the soybean full-length-enriched library.
Because 3' sequence is used to select probes, we aligned (BLAST) RefSeqs with all human mRNA sequences from the GenBank Primate (PRI) and high-throughput cDNA sequencing (HTC) division to extend the 3' end of the transcripts as far as possible.
To extend the microarray meta-analysis, transcript accumulation of the CRK subfamily was monitored in response to O3 and light stress by qPCR.
For NC-350, Northern analyses suggested that two of the four NC-350 transcripts extend the coding sequence of gene slr0551 and therefore might constitute its 5′-UTR rather than be a bona fide sRNA.
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CEO of Professional Science Editing for Scientists @ prosciediting.com