Human Pol κ inserted dCMP opposite the exocyclic lesions less efficiently than hPol ι did, but hPol κ was able to extend template-primers containing dNMPs opposite the adducts.
The present results demonstrate that hPol κ, hPol ι, and Rev1 are able to insert deoxynucleotides opposite M1dG and that hPol κ is able to extend template-primers containing deoxynucleotides opposite the lesion.
This extends template stripping to high-melting-point solids, such as W, and transparent conductors, such as ITO, both of which are difficult to pattern with conventional methods.
The SMART™ oligonucleotide which has an oligo(G) sequence at its 3′ end, base-pairs with the deoxycytidine stretch, creating an extended template.
It is hypothesized that clinical lymph node relapse could be the direct consequence of a suboptimal PLND at the initial treatment or a progression outside the boundaries of the standard extended template.
hPol κ extended template-primers in the order M1dG dC > M1dG dG > M1dG dT ∼ M1dG dA, but neither hPol ι nor Rev1 extended M1dG-containing template-primers.
Support for this hypothesis is provided by the observation that hPol κ is very inefficient at extending template-primers containing dC PdG termini (27).
Single nucleotide incorporation experiments indicate that hPol κ extended template-primers containing dC opposite M1dG approximately 10-fold more efficiently than template-primers containing other deoxynucleotides opposite the adduct when it is contained in the 3′-GXC-5′ sequence.
Briefly, this protocol consisted of incubating the protein lysate with an artificial telomerase template, allowing the telomerase to extend the template.
If the Democrats try to extend the template to multilateral trade, Doha's prospects, already bleak, darken further.
Also, we are planning to extend the template to include serotonin transporters.
