Further studies are needed to extend our database of transcripts whose levels of expression are reliably concordant between brain and blood.
Recent reports using shRNA or siRNA-containing lentiviruses [ 27] suggest that our scheme could also be used for knock-down of ligand transcripts to extend our understanding of the factors that regulate the intestinal stem cell or other organ stem cells that are modulated by paracrine signaling.
Sequencing 1600 clones from one of the libraries confirmed and extended our results to endogenous transcripts and demonstrated that some very abundant transcripts common to tester and driver escaped subtraction.
Bioinformatic analysis coupled with gene transcript profiling extends our understanding of the iron and reduced inorganic sulfur compounds oxidation pathways in A. ferrooxidans.
Bioinformatic analysis coupled with gene transcript profiling extends our understanding of the iron and reduced inorganic sulfur compounds oxidation pathways in A. ferrooxidans and suggests mechanisms for their regulation.
Next, we extended our study to the primary transcripts of lin-4, mir-237, mir-48, and mir-77, none of which has been reported to undergo trans-splicing, and for none of which we could amplify a trans-spliced product using RT PCR with an SL1-specific primer and a pri-miRNA specific primer (data not shown).
Due to the use of similarity information between two related organisms to extend transcripts, our algorithm cannot identify extended transcripts that are not shared between the two organisms.
Whereas RefSeq extend all transcripts at a locus sharing the same first and final exon to use the same transcription start and end site, GENCODE only extend a transcript as far as the supporting evidence allows.
In the present study we focus on further analysis of transcript biomarkers in predicting response to RTX in order to determine the value of the proclaimed transcript markers and extend the transcript profile associated with response outcome.
Western blot analysis of 22 DAP W64A + and W64A o2 maize endosperm was performed to extend the transcript profiling and proteomic analysis; quantitative measurement by densitometry is shown in Table 2.
Here, we used the Medicago GeneChip® to extend the transcript dataset for mycorrhizal roots and coupled this with LM and RT-PCR to assess gene expression specifically at the cortical cell-arbuscule interface.
