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In relation to operational issues, LRE provides several attributes that facilitate large-scale absolute quantification, with the potential to extend assay performance to include the general concept of assay robustness.
SNP typing was performed with the Homogeneous Mass Extend assay (Sequenom, Hamburg, Germany).
We designed the Multiplexed SNP Mass EXTEND assay using Sequenom MassARRAY Assay Design version 4.0 software [ 14].
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Low Z′ factors, narrow dynamic ranges, and/or extended assay times are commonly reported in current gametocyte cell-based assays measuring gametocyte-expressed fluorescent or luciferase reporters, endogenous ATP levels, activity of gametocyte enzymes, or redox-dependent dye fluorescence.
Low Z′ factors, narrow dynamic ranges, and/or extended assay times are commonly reported in current gametocyte assays measuring gametocyte-expressed fluorescent or luciferase reporters, endogenous ATP levels, activity of gametocyte enzymes, or redox-dependent dye fluorescence.
In the present study we extend the assay to the identification of 11 vertebrate species, and the parasite T. cruzi using species-specific PCR assays.
The neutralization results obtained so far, using the IVIG preparation, prompted us to extend the assay application.
This suggests that fluoromycobacteriophages can be used to evaluate INH susceptibilities although the requirement for pre-incubation with Tween may extend this assay to longer than 48 hours.
To extend our assay in vivo, Tau-seeds were injected in frontal cortex and hippocampus of TauP301S mice and analyzed for Tau-aggregation in vivo.
Megascript kit (Ambion) and poly dT) 3' primer with a 3-fold degenerate (A/G/C) 3' end (for site of mRNA cleavage assay), poly dT) 3' primer (for PAT assay), or random hexamers (for extended transcript assay) were used to prepare cDNA.
We consequently decided to use a dedicated kit to re-extract DNA from FFPE tissues and to extend the assays to the two other markers.
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